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Particle and Surface Sciences are the sole distributors throughout Australia and New Zealand for the Nanosight range of products. PsSâ„¢ would like to bring to your attention the latest news and technology regarding the Nanosight systems. 

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NanoSight's Technology

This is not Light Scattering, this is NanoSight. This is neither Dynamic Light Scattering (DLS) nor Photon Correlation Spectroscopy (PCS), it is Nanoparticle Tracking Analysis (NTA). In common they have laser light, Brownian motion and Stokes Einstein. Light Scattering (DLS or PCS) assesses the time-based corruption of coherent light to produce an average particle size, necessarily biased to the larger particles. In contrast, NanoSight simultaneously measures the speed of many observed particles and compiles these particle-by-particle into a much higher resolution particle size distribution than DLS/PCS, so much better reflecting the complexity of real-world samples.

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Nanoparticle Tracking Analysis (NTA) is a relatively new technique for the rapid, direct and real-time visualization of nanoparticles in a liquid. Based on laser-illuminated optical microscopy, nanoparticles are seen as light-scattering centers moving under Brownian motion. A video of the movement of each particle is analyzed on a frame-by-frame basis and the particle's size is established from an analysis of the average speed of movement. Because each particle is measured separately, it is possible to measure particle size and relative light-scattering intensity at the same time, allowing better resolution of mixtures of different particle sizes or types within a sample than would be possible with conventional dynamic light-scattering techniques such as photon correlation spectroscopy. Importantly, particle count or concentration are also measured.

nanotox Nanoparticle Toxicology

Particle size, concentration and degree of aggregation all influence the biological response to nanoparticles and the concomitant potential health implications. NanoSight provides these measurands at high-resolution with visual validation of the data. In fluorescence modes NTA can differentiate labeled-particles from complex background, with an ability to detect individual quantum dots.

lipo Drug Delivery

Particle size distribution is at the heart of realizing the benefits of targeted drug delivery. NanoSight provides a high-resolution particle size distribution and concentration measurement across the range of sizes employed in delivery vehicles such as liposomes and polymeric nanoparticles.

virus Virology and Vaccine Production

Counting and sizing viruses, bacteriophage and their aggregates is important in the development and validation of vaccines and phage therapeutics. NanoSight provides total viral count in just minutes, providing a rapid alternative to traditional methods measuring infective titer.

eco Ecotoxicology

Understanding the fate, particle aggregation, size and concentration of engineered nanoparticles in the natural environment are key parameters in ecotoxicology. NanoSight's ability to measure concentrations down to 107 particles per ml, to count aggregates and to operate in fluorescence mode make it ideally suited to this area of research.

proagg Protein Aggregation

Protein aggregation can occur during many unit operations in the biological manufacturing processes: cell culture, purification, formulation, packing and storage. Determining the state and kinetics of aggregation can improve product stability and provide significant gains in process optimization. NanoSight provides sizing and count of protein aggregates in the region of 30 -

1000nm.

ortho Orthopedic Implants

The mechanical action of orthopedic implants moving against each other in situ, and the corrosive action of the body, cause implants to generate wear debris. NanoSight can provide particle size and concentration of wear debris in liquid suspension in minutes and can differentiate populations of metallic, ceramic and polymeric particles.

inks Paints and Pigments

Particle size and concentration are important features in inkjet applications. Particle size influences the strength of colour and particle aggregates may also cause nozzle blockage. The high-resolution size distributions, as generated by NanoSight, are proving to be key in this application.

nanobubbles Nanobubbles

Nanobubbles have a wide range of applications including drug delivery, cleaning and sterilization. NanoSight can visualize nanobubbles directly in solution and provide information on bubble size and concentration.

Please contact PsS for prices and latest brochures of our product range.  Particle & Surface Sciences are also the sole distributors throughout Australia and New Zealand for Dataphysics, DOP Solutions, Micromeritics, Lighthouse Worldwide Solutions, NanoSight Technology, Clemex, Ankersmid, Impact Test equipment, Mipoy and their own products, PsS POLA 2000, POLA 3000 particle counters.   

 For technical advice for your particular application, please call or email us in return and we will be happy to assist you.

Particle & Surface Sciences Pty Limited
P O Box 1926
Unit 4/31 Dwyer Street
North Gosford   New South Wales   2250
Australia

Ph:   +61 02 4323 7822
Fax:  +61 02 4323 7629

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10th September 2008

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Particle and Surface Sciences are pleased to announce the launch of NanoSight Technology.  NanoSight will provide a new range of products to enhance and broaden the range of particle characterization systems already offered by Particle and Surface Sciences Australia.

NanoSight provides a unique technology to visualise and size nano-scale particles in liquid down to 10 nm with little preparation and at low cost. Based on a fundamental understanding of existing particle sizing techniques, the NanoSight technology has been developed into a unique and exciting instrument for nanoparticle sizing, available as both a research instrument and in a bench-top format.

The Nanoparticle Tracking Analysis (NTA) technique is based on laser light scattering microscopy. The instruments can visualise and dynamically size populations of particles in a liquid on an individual basis in the particle size range of 10 nm – 1000 nm (dependent on material). The Brownian motion of each and every particle (which appear as point scatterers) is tracked separately but simultaneously using a CCD camera, from which a high-resolution plot of the particle size distribution profile (and changes therein in time) is obtained.

This particle-by-particle approach avoids the averaging assumptions of Photon Correlation Spectroscopy (PCS, also known as Dynamic Light Scattering, DLS) and provides a unique image, going beyond PCS in assessing polydisperse systems. The technique uniquely allows the user a simple and direct qualitative view of the sample under analysis (perhaps to validate data obtained from other techniques such as PCS) and from which an independent quantitative estimation of sample size, size distribution and concentration can be immediately obtained. Sample pre-treatment is minimal, requiring only dilution with a suitable solvent to an acceptable concentration range (between 108 and 109 particles per ml depending on sample type). All particle types can be measured and in any solvent type providing that the particles scatter sufficient light to be visible (i.e. are not index matched).

The NanoSight NTA technique represents an attractive alternative or complement to higher cost and more established methods of nanoparticle analysis such as photon correlation spectroscopy or electron microscopy that are currently employed in a wide range of technical and scientific sectors.  

Particle & Surface Sciences () also offer a wide-ranging programme of support solutions, including installation and service of all instruments by factory-trained technicians, an Analytical Laboratory and a Sales Department to assist with your enquiries.  PsSâ„¢ will be running a series of seminars across Australia during the month of February 2009.  Please visit the PsSâ„¢ website for more information on the locations.
Last Updated on Wednesday, 19 January 2011 09:22  

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